TRIM antibody

• Catalog Number: orb44526
• Category: Antibodies
• Description: Mouse Monoclonal to TRIM.
• Clonality: Monoclonal
• Clone Number: TRIM-04
• Tested applications: FC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant intracellular domain (aa 29-186) of human TRIM.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining. Western blotting: Non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: TRIM
• Entrez: 50852
• UniProt ID: Q6PIZ9
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: TRIM antibody, anti-T cell receptor-interacting mo
• Note: For research use only
• Application notes: Flow cytometry: Intracellular staining. Western blotting: Non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb TRIM-04 and goat anti-mouse IgG (H+L)-HRP conjugate.

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-TRIM (TRIM-04) purified antibody (concentration in sample 1 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD3 positive TRIM positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-TRIM (TRIM-04) purified antibody (concentration in sample 1 µg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-TRIM (TRIM-04) purified antibody (concentration in sample 1 µg/ml, GAM APC).