TRIM Antibody (PE)

• Catalog Number: orb44527
• Category: Antibodies
• Description: Anti-TRIM PE
• Clonality: Monoclonal
• Clone Number: TRIM-04
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant intracellular domain (aa 29-186) of human TRIM.
• Concentration: 0.1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: TRIM
• Entrez: 50852
• UniProt ID: Q6PIZ9
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: TRAT, TCRIM
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human lymphocytes using anti-human CD3 (UCHT1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood) and intracellular staining of human lymphocytes using anti-TRIM (TRIM-04) PE antibody (concentration in sample 1 µg/ml).

Comparison of anti-TRIM (TRIM-04) PE antibody nonspecific intracellular staining (red) and mouse IgG2a Isotype control (MOPC-173) PE antibody nonspecific intracellular staining (concentration in sample 5 µg/ml, same as anti-TRIM PE concentration, blue) co-stained by anti-mouse CD3 (145-2C11) APC antibody (concentration in sample 1.2 µg/ml) in flow cytometry analysis of murine splenocyte suspension. Clone TRIM-04 is not specific for murine TRIM.

Separation of TRIM positive CD3 positive human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-TRIM (TRIM-04) PE antibody (concentration in sample 1 µg/ml).

Comparison of anti-TRIM (TRIM-04) PE antibody nonspecific staining (blue-filled) and mouse IgG2a Isotype control (MOPC-173) PE antibody nonspecific staining (concentration in sample 5 µg/ml, same as anti-TRIM PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of murine splenocyte suspension. Clone TRIM-04 is not specific for murine TRIM.

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-TRIM (TRIM-04) PE antibody (concentration in sample 1 µg/ml).