TRF1 Rabbit Polyclonal Antibody

SKU: orb11511

Description

TRF1 Rabbit Polyclonal Antibody

Research Area

DNA and RNA Research, Telomeres

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TRBF1 (121-220/439aa)
• Target: TERF1
• Molecular Weight: 50 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

hTRF1 AS; NIMA interacting protein 2; NIMA-interacting protein 2; PIN 2; PIN2; t TRF1; Telomeric protein Pin2; Telomeric protein Pin2/TRF1; Telomeric repeat binding factor(NIMA interacting) 1; Telomeric repeat binding factor 1; Telomeric repeat binding protein 1; Telomeric repeat-binding factor 1; TERF 1; TERF1; TERF1_HUMAN; TRBF 1; TRBF1; TRF 1; TRF; TTAGGG repeat binding factor 1; TTAGGG repeat-binding factor 1.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TRF1) Polyclonal Antibody, Unconjugated (orb11511) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: HL-60 (Human) Cell Lysate at 30 ug, Primary: Anti-TRF1 (orb11511) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 49 kD.

Sample: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-TRF1 (orb11511) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 49 kD.

Quick Database Links

Gene Symbol

TERF1

UniProt

P54274