TREM2 Rabbit Polyclonal Antibody

• Catalog Number: orb313249
• Category: Antibodies
• Description: TREM2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Human
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TREM2
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35-40 kDa
• Target: TREM2
• UniProt ID: Q9NZC2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Triggering receptor expressed on myeloid cells 2;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Cao, Cheng et al. TREM2 modulates neuroinflammation with elevated IRAK3 expression and plays a neuroprotective role after experimental SAH in rats Neurobiol Dis, 171, 105809 (2022)

PubMed: 35781003

Applications

IF

Reactivity

Rat

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated (orb313249) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated (orb313249) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated (orb313249) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Lane 1: Mouse Raw264.7 cell lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Mouse Cerebrum tissue lysates, Lane 4: Mouse BV2 cell lysates, Lane 5: Mouse Lung tissue lysates, Lane 6: Rat Spleen tissue lysates, Lane 7: Rat Cerebrum tissue lysates, Primary: Anti-TREM2 (orb313249) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kDa, Observed band size: 24 kDa.

Sample: Lane 1: Mouse Spleen tissue lysates, Lane 2: Mouse Raw264.7 cell lysates, Lane 3: Mouse BV2 cell lysates, Lane 4: Human HepG2 cell lysates, Primary: Anti-TREM2 (orb313249) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kDa, Observed band size: 26 kDa.