TREM1 Rabbit Polyclonal Antibody

• Catalog Number: orb158644
• Category: Antibodies
• Description: TREM1 Rabbit Polyclonal Antibody
• Target: TREM1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human TREM1 (151-234/234aa)
• UniProt ID: Q9NP99
• MW: 24 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Triggering receptor expressed on myeloid cells 1;
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TREM1) Polyclonal Antibody, Unconjugated (orb158644) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Primary Antibody: Rabbit anti-TREM1 antibody, catalog number: orb158644, Isotype Control Antibody: Rabbit IgG, Secondary Antibody: F (ab')2 fragment goat anti-rabbit IgG-PE. Positive control: U937 (4% paraformaldehyde for 10 min at room temperature).

Primary Antibody: Rabbit anti-TREM1 antibody, catalog number: orb158644, Isotype Control Antibody: Rabbit IgG, Secondary Antibody: F (ab')2 fragment goat anti-rabbit IgG-PE. Positive control: U937 (4% paraformaldehyde for 10 min at room temperature).

Sample: hcclm3 (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, Primary: Anti-TREM1 (orb158644) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 21 kD.