TREF1 Antibody (C-term)

SKU: orb1427372

Description

Rabbit polyclonal antibody to TREF1.

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB24740
• Immunogen: Synthetic Peptide
• Target: TRERF1
• Molecular Weight: 132256
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transcriptional-regulating factor 1, Breast cancer anti-estrogen resistance 2, Transcriptional-regulating protein 132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132, TRERF1, BCAR2, RAPA, TREP132

Fluorescent image of MCF-7 cell stained with TREF1 Antibody (C-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TREF1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). TREF1 immunoreactivity is localized to Nucleus significantly.

TREF1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of TREF1 Antibody (C-term) in K562 cell line lysates (35 ug/lane). TREF1 (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

TRERF1

UniProt

Q96PN7

RefSeq (Protein):NP_277037.1