TRAP80 Rabbit Polyclonal Antibody

• Catalog Number: orb156454
• Category: Antibodies
• Description: TRAP80 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TRAP80 (181-280/651aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 73 kDa
• Target: MED17
• UniProt ID: Q9NVC6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: MED17; CRSP77; Activator recruited cofactor 77 kDa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Hela (fixed with pre-warmed 4% paraformaldehyde for 30 min at 37°C and then permeabilized with 90% ice-cold methanol for 30 min on ice), Primary Antibody (green line): Rabbit Anti-TRAP80 antibody (orb156454), ilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test.

Sample: MDA-MB-231 Cell (Human) Lysate at 40 ug, Primary: Anti-TRAP80 (orb156454) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 73 kD, Observed band size: 73 kD.

Sample: spleen (mouse) Lysate at 40 ug, Primary: Anti-TRAP80 (orb156454) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 73kD, Observed band size: 75 kD.