Transglutaminase 2 Rabbit Polyclonal Antibody

• Catalog Number: orb2986
• Category: Antibodies
• Description: Transglutaminase 2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Transglutaminase 2 (351-450/687aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 77 kDa
• Target: TGM2
• UniProt ID: P21980
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: TG 2; TG(C); TG2; TGase C; TGase H; TGase-2; TGase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Shawgi Hago Almugadam 1 2, Alessandro Trentini 3, Martina Maritati 4, Carlo Contini 4, Maria Cristina Manfrinato 1, Carlo Cervellati 5, Tiziana Bellini 1, Stefania Hanau 1 A Calcium- and GTP-Dependent Transglutaminase in Leishmania infantum Vet Sci, 10, 234 (2023)

PubMed: 36977273

Applications

IF, WB

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TGM2) Polyclonal Antibody, Unconjugated (orb2986) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human Pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TGM2) Polyclonal Antibody, Unconjugated (orb2986) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Uterus (Mouse) Lysate at 40 ug, Primary: Anti-Transglutaminase 2 (orb2986) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 77 kD, Observed band size: 74 kD.