TPOR Rabbit Polyclonal Antibody

• Catalog Number: orb186269
• Category: Antibodies
• Description: TPOR Rabbit Polyclonal Antibody
• Target: MPL
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human TPOR (401-500/635aa)
• UniProt ID: P40238
• MW: 68 kDa
• Tested applications: FC
• Dilution range: Flow-Cyt=3μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: C MPL; C-MPL; CMPL; CD110; CD 110; MPL; MPLV; Myel
• Note: For research use only

Blank control (Black line): U2OS. Primary Antibody (green line): Rabbit Anti-TPOR antibody (orb186269), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% paraformaldehyde for 10 min at room temperature. Cells incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were then stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.