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Catalog Number | orb186269 |
---|---|
Category | Antibodies |
Description | TPOR Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC |
Predicted Reactivity | Canine, Mouse, Rat, Sheep |
Reactivity | Human |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human TPOR (401-500/635aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | Flow-Cyt=3μg/Test |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 68 kDa |
Target | MPL |
UniProt ID | P40238 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | C MPL; C-MPL; CMPL; CD110; CD 110; MPL; MPLV; Myel Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control (Black line): U2OS. Primary Antibody (green line): Rabbit Anti-TPOR antibody (orb186269), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% paraformaldehyde for 10 min at room temperature. Cells incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were then stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
FC, WB | |
Canine, Mouse, Rat, Sheep | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC | |
Canine, Mouse, Rat, Sheep | |
Human | |
Rabbit | |
Polyclonal | |
APC |