TPI1 Antibody

• Catalog Number: orb1264514
• Category: Antibodies
• Description: TPI1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Monkey, Porcine, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human TPI1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 31 kDa
• Target: TPI1
• UniProt ID: P60174
• NCBI: P60174
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Triosephosphate isomerase, TIM, Triose-phosphate i
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of TPI1 Antibody in Y79 (lane 1), CEM (lane 2) cell line and mouse brain tissue (lane 3) lysates (35 ug/lane) (2ug/ml)

Western blot analysis of TPI1 using rabbit polyclonal TPI1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPI1 gene.

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.