Topo II Beta Antibody

• Catalog Number: orb1529342
• Category: Antibodies
• Description: Topo II Beta Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 1475 and 1525
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Topo II Beta
• UniProt ID: Q02880
• NCBI: NP_001059.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: topo II beta; antigen MLAA-44; topoisomerase IIb;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Topo II Beta by western blot.Samples: Whole cell lysate from HEK293T, Jurkat, K-562, MCF-7, and A-549 cells. Antibody: Affinity purified rabbit anti-Topo II Beta antibody at 0.04 µg/ml.

Detection of human Topo II Beta by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:200 (1µg/ml).

Detection of human Topo II Beta by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-Topo II Beta antibody.