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Catalog Number | orb1529340 |
---|---|
Category | Antibodies |
Description | Topo II Beta Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | between 1570 and C-term |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 to 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Topo II Beta |
UniProt ID | Q02880 |
NCBI | NP_001059.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | topo II beta; antigen MLAA-44; topoisomerase IIb; Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse Topo II Beta by western blot.Samples: Whole cell lysate from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-Topo II Beta antibody orb1529340 at 0.04 µg/ml.
Detection of human Topo II Beta by immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:1, 000 (0.2µg/ml).
Detection of human Topo II Beta by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:1, 000 (0.2µg/ml).
Detection of human Topo II Beta by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-Topo II Beta antibody.
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