Toll-like Receptor 7/TLR7 Antibody

• Catalog Number: orb1294285
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Toll-like Receptor 7
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human Toll-like Receptor 7 / TLR7
• Dilution range: Western Blot 1:500-1:1500 Immunofluorescence 1:200-1:400 Immunohistochemistry (Paraffin) 1:200-1:300
• Conjugation: Unconjugated
• Target: Toll-like Receptor 7 / TLR7
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Toll-like Receptor 7 antibody at 1/800 dilution, Lysates at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294285 overnight at 4°C.

Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294285 Anti-Toll-like Receptor 7 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse testis labeling Toll-like Receptor 7 with orb1294285 at 1/200.