TLR7 Antibody

• Catalog Number: orb259629
• Category: Antibodies
• Description: TLR7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TLR7 (1020-1047aa QAHPYFWQCLKNALATDNHVAYSQVFKE), different from the related mouse sequence by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, - Immunocytochemistry , 0.5-1μg/ml, Human, - Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 120922 MW
• UniProt ID: Q9NYK1
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Toll-like receptor 7;TLR7;UNQ248/PRO285;
• Note: For research use only
• Application notes: WB: The detection limit for TLR7 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of H-PBMC cells using anti-TLR7 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of TLR7 using anti-TLR7 antibody.Lane 1:MCF-7 cell;2:COLO320 cell;3:JURKAT Cell.

IHC analysis of TLR7 using anti-TLR7 antibody.TLR7 was detected in paraffin-embedded section of mouse thymus tissue.

IHC analysis of TLR7 using anti-TLR7 antibody.TLR7 was detected in paraffin-embedded section of rat thymus tissue.

IHC analysis of TLR7 using anti-TLR7 antibody.TLR7 was detected in paraffin-embedded section of human placenta tissue.

IHC analysis of TLR7 using anti-TLR7 antibody.TLR7 was detected in frozen section of human placenta tissue.

IHC analysis of TLR7 using anti-TLR7 antibody.TLR7 was detected in immunocytochemical section of a549 cell.