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Catalog Number | orb1294285 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Toll-like Receptor 7 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / Intracellular domain corresponding to Human Toll-like Receptor 7 / TLR7 |
Dilution range | Western Blot 1:500-1:1500 Immunofluorescence 1:200-1:400 Immunohistochemistry (Paraffin) 1:200-1:300 |
Conjugation | Unconjugated |
Target | Toll-like Receptor 7 / TLR7 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-Toll-like Receptor 7 antibody at 1/800 dilution, Lysates at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294285 overnight at 4°C.
Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294285 Anti-Toll-like Receptor 7 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded mouse testis labeling Toll-like Receptor 7 with orb1294285 at 1/200.
FC, ICC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC | |
Human, Monkey, Mouse | |
Monoclonal | |
Unconjugated |
Greater than 85% as determined by SDS-PAGE. | |
43.8 kDa | |
E.coli |