TNFSF18 Antibody

• Catalog Number: orb1272727
• Category: Antibodies
• Description: TNFSF18 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hAITRL. Human AITRL specific antibody was purified by affinity chromatography employing immobilized hAITRL matrix.
• Concentration: batch dependent
• Dilution range: ELISA:Indirect:To detect hAITRL by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hAITRLSandwichTo detect hAITRL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human AITRL as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hAITRL. Western Blot:To detect hAITRL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAITRL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: TNFSF18
• UniProt ID: Q9UNG2
• NCBI: Q9UNG2
• Storage: AITRL antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: TL6, AITRL, GITRL, hGITRL, TL6, UNQ149/PRO175, Tum
• Note: For research use only
• Application notes: ELISA:Indirect:To detect hAITRL by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hAITRLSandwichTo detect hAITRL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human AITRL as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hAITRL. Western Blot:To detect hAITRL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAITRL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect hAITRL by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human AITRL (orb1272726) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hAITRL.

To detect hAITRL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAITRL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hAITRL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAITRL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained A-431 cells. The primary antibody was incubated at 3.0 ug/ml overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary.