TNFSF14 Rabbit Polyclonal Antibody

SKU: orb13725

Description

TNFSF14 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:500-5000
• Reactivity: Mouse
• Predicted Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TNFSF14 (141-240/240aa)
• Target: TNFSF14
• Molecular Weight: 26 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Tumor necrosis factor ligand superfamily member 14; CD 258; CD258; CD258 antigen; delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator A; Herpesvirus entry mediator ligand; herpesvirus entry mediator-ligand; HVEM L; HVEM-L; HVEML; Ligand for herpesvirus entry mediator; LIGHT; LTg; soluble form; TNF14; TNFSF 14; TNFSF14 protein; TR 2; TR2; Tumor necrosis factor(ligand) superfamily, member 14; Tumor necrosis factor ligand superfamily, member 14; Tumor necrosis factor receptor like 2; Tumor necrosis factor superfamily member LIGHT; TNF14_HUMAN.

Sample: Spleen (Mouse) Lysate at 40 ug, Small intestine (Mouse) Lysate at 40 ug, Primary: Anti-TNFSF14 (orb13725) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 26 kD.

Tissue/Cell: Mouse liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TNFSF14 Polyclonal Antibody, Unconjugated (orb13725) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

TNFSF14

UniProt

O43557

References

Lai, Dongming et al. LIGHT Amplification by NF-?B Contributes to TLR3 Signaling Pathway-Induced Acute Hepatitis Mediators Inflamm, 2023, 3732315 (2023)

PubMed: 36654880