TNFRSF25 Antibody

• Catalog Number: orb1239336
• Category: Tools
• Description: TNFRSF25 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Isotype: IgG
• Immunogen: DR3 antibody was raised against a peptide corresponding to amino acids in extracellular domain of human DR3 precusor.The immunogen is located within amino acids 50 - 100 of DR3.
• Concentration: 1 mg/ml
• Dilution range: DR3 antibody can be used for detection of DR3 expression by Western blot at 1 μg/mL. 59 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 59 kDa
• Target: TNFRSF25
• UniProt ID: Q93038
• NCBI: AAQ88676
• Storage: DR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: DR3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: DR3 Antibody: DR3, TR3, DDR3, LARD, APO-3, TRAMP,
• Note: For research use only
• Application notes: DR3 antibody can be used for detection of DR3 expression by Western blot at 1 μg/mL. 59 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:1000 dilution.

Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 µg/mL.

Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 µg/mL.

Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 µg/mL. Green: DR3 Antibody (orb1239336) Blue: DAPI staining.

Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 µg/mL.