Tnfrsf18 Antibody

• Catalog Number: orb1671762
• Category: Antibodies
• Description: Tnfrsf18 Antibody
• Clonality: Recombinant
• Clone Number: DTA-1
• Tested applications: FC, Functional Studies, IP, WB
• Reactivity: Mouse
• Isotype: IgG lambda
• Immunogen: This antibody was raised by immunising Wistar rats with the CD25+CD4+ T cell line. Hybridoma cells were generated by fusing spleen cells from the immunized rat with P3X63Ag8.653 myeloma cells, assessed for secreting antibodies capable of neutralizing in vitro CD25+CD4+ T cell-mediated suppression of anti-CD3-stimulated T cell proliferation9, and then cloned. This DTA-1 clone secreted a monoclonal rat immunoglobulin G2a (IgG2a) that showed high neutralizing activity.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Tnfrsf18
• UniProt ID: O35714
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD357, AITR, GITR-D, Glucocorticoid-induced TNFR-r
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed mouse splenocytes with anti-GITR antibody DTA-1. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of DTA-1 (orb1671762) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671762- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-GITR antibody DTA-1. Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of DTA-1 (orb1671762 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.