GITR/TNFRSF18 Antibody

• Catalog Number: orb1519833
• Category: Antibodies
• Description: GITR/TNFRSF18 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human
• Immunogen: between 191 and 241 (C-term)
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1000; IP - 6 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: GITR/TNFRSF18
• UniProt ID: Q9Y5U5
• NCBI: NP_004186.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: GITR-D; tumor necrosis factor receptor superfamily
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human GITR/TNFRSF18 by western blot. Samples: Whole cell lysate (15 or 50 µg) from MJ, Jurkat, RPMI-8226, HeLa, Raji, MOLT-4, Daudi, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (orb1519833) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-ß-Actin recombinant monoclonal antibody.

Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (orb1519833) used at 1:125. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (orb1519833) used at 1:200. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).