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TNFAIP3 interacting protein 3 Rabbit Polyclonal Antibody

SKU: orb100196
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Description

TNFAIP3 interacting protein 3 Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityMouse, Rat
Predicted ReactivityBovine, Human, Sheep

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human TNFAIP3 interacting protein 3/ABIN3 (51-150/325aa)
TargetTNIP3
Molecular Weight39 kDa
PurificationAffinity purified by Protein A

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

Listeria induced; A20-binding inhibitor of NF-kappa-B activation 3; ABIN-3; ABIN-3 beta; FLJ21162; LIND; Listeria induced gene protein; TNFAIP3 interacting protein 3; TNFAIP3-interacting protein 3 beta; TNIP3; TNIP3 beta; TNIP3_HUMAN.

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TNFAIP3 interacting protein 3 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (TNFAIP3 interacting protein 3) Polyclonal Antibody, Unconjugated (orb100196) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

TNFAIP3 interacting protein 3 Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Adrenal gland tissue lysates, Lane 3: Mouse Kidney tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Rat Spleen tissue lysates, Lane 6: Rat Adrenal gland tissue lysates, Lane 7: Rat Kidney tissue lysates, Primary: Anti-TNFAIP3 interacting protein 3 (orb100196) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kDa, Observed band size: 40 kDa.

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC-P
Immunohistochemistry Paraffin
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IHC-Fr
Immunohistochemistry Frozen
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IF
Immunofluorescence
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TNFAIP3 interacting protein 3 Rabbit Polyclonal Antibody (orb100196)

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50 μl
$ 240.00
100 μl
$ 390.00
200 μl
$ 510.00
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