TMEM173 Antibody

• Catalog Number: orb1239632
• Category: Antibodies
• Description: TMEM173 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine, Gallus, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-MPYS antibody (orb1239632) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human MPYS. The immunogen is located within amino acids 260-310 of MPYS.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 42kDObserved: 42 kD
• Target: TMEM173
• UniProt ID: Q86WV6
• NCBI: NP_938023
• Storage: MPYS antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: MPYS Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: MPYS Antibody: ERIS, MITA, MPYS, NET23, STING, ERI
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: MPYS orb1239632 (1 µg/mL), MPYS orb1271001 (2 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: MPYS orb1239632 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human THP-1 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: MPYS orb1239632 (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse A-20 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: MPYS orb1239632 (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry Validation of MPYS in Mouse A20 Cells. Immunocytochemical analysis of A20 cells using anti-MPYS antibody (orb1239632) at 5 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of MPYS in Moue A20 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed A20 Cells labeling MPYS with orb1239632 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).