TMEM158 Rabbit Polyclonal Antibody

• Catalog Number: orb158619
• Category: Antibodies
• Description: TMEM158 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Gallus, Human, Porcine
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TMEM158 (188-260/300aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 28 kDa
• Target: TMEM158
• UniProt ID: Q8WZ71
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 40 kDa BINP binding protein; 40 kDa BINP-binding p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Mouse heart cells (blue). Primary Antibody: Rabbit Anti-TMEM158 antibody (orb158619), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Blank control: Mouse spleen cells (blue). Primary Antibody: Rabbit Anti-TMEM158 antibody (orb158619), dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TMEM158) Polyclonal Antibody, Unconjugated (orb158619) at 1:400 overnight at 4°C, followed by a conjugated secondary (orb868589) at 1:500 for 90 minutes and DAPI staining of the nuclei.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TMEM158) Polyclonal Antibody, Unconjugated (orb158619) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TMEM158 Polyclonal Antibody, Unconjugated (orb158619) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.