TM7SF2 Rabbit Polyclonal Antibody

• Catalog Number: orb4574
• Category: Antibodies
• Description: TM7SF2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TM7SF2/DHCR14A (51-150/418aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 46 kDa
• Target: TM7SF2
• UniProt ID: O76062
• RRID: AB_10926586
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ANG1; Another new gene 1 protein; DHCR14A; C 14 st
• Note: For research use only
• Expiration Date: 12 months from date of receipt

References

Xu, Yichi et al. TM7SF2 regulates cell proliferation and apoptosis by activation of C-Raf/ERK pathway in cervical cancer Cell Death Discov, 7, 299 (2021)

PubMed: 34667152

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (Human adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (TM7SF2) Polyclonal Antibody, Unconjugated (orb4574) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (TM7SF2) Polyclonal Antibody, Unconjugated (orb4574) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human prostatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (TM7SF2) Polyclonal Antibody, Unconjugated (orb4574) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Adrenal gland tissue lysates, Lane 2: Rat Adrenal gland tissue lysates, Primary: Anti-TM7SF2 (orb4574) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kDa, Observed band size: 58 kDa.