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Catalog Number | orb1252891 |
---|---|
Category | Antibodies |
Description | TLR9 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | TR9541 |
Tested applications | FC, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG1, kappa |
Immunogen | A recombinant protein fragment from the N-terminal portion of human TLR9 was used as the immunogen for this antibody. |
Concentration | 0.5 mg/mL |
Dilution range | Western blot: 2-4 ug/mlFlow Cytometry: 1ug/10e6 cellsImmunohistochemistry (Paraffin): 3-5 ug/ml (1)1. For optimal TLR9 staining, formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | TLR9 |
UniProt ID | Q9NR96 |
Storage | Aliquot and Store at -20°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | TLR9 Antibody: CD289, Toll-like receptor 9 Read more... |
Note | For research use only |
Application notes | Western blot: 2-4 ug/mlFlow Cytometry: 1ug/10e6 cellsImmunohistochemistry (Paraffin): 3-5 ug/ml (1)1. For optimal TLR9 staining, formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. |
Expiration Date | 12 months from date of receipt. |
Western blot testing of human samples with TLR9 antibody at 2 ug/ml.
Western blot testing of mouse samples with TLR9 antibody at 2 ug/ml.
IHC testing of human tonsil stained with TLR9 antibody at 5 ug/ml.
ELISA, IF, IHC-P, WB | |
Bovine, Porcine, Sheep | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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