TLR4 Rabbit Polyclonal Antibody

SKU: orb500827

Description

TLR4 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-2000, IHC-P=1:100-500, IHC-F=1:500-1000, IF=1:200-1000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TLR4 (701-800/839aa)
• Target: TLR4
• Molecular Weight: 90 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

TLR-4; TLR 4; ARMD10; CD 284; CD284; CD284 antigen; Homolog of Drosophila toll; hTol; Toll(Drosophila) homolog; TOLL; Toll Endotoxin Hyporesponsiveness; Toll like receptor 4; Toll like receptor 4 precursor; TLR4_HUMAN.

4% Paraformaldehyde fixed PC12 cells stained with Rabbit Anti-TLR4 Polyclonal Antibody (orb500827) at 1:300 for 3 hours at 4°C, followed by Alexa Fluor 488-conjugated secondary antibody for an additional hour.

Sample: K562 (Human) Cell Lysate at 40 ug, HL-60 (Human) Cell Lysate at 40 ug, RAW264.7 (Mouse) Cell Lysate at 40 ug, Primary: Anti-TLR4 (orb500827) at 1/2000 dilution.

Quick Database Links

Gene Symbol

TLR4

UniProt

O00206