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TLR4 Rabbit Polyclonal Antibody (FITC)

Catalog Number: orb463501

DispatchUsually dispatched within 2-3 weeks
$ 450.00
Catalog Numberorb463501
CategoryAntibodies
DescriptionTLR4 Rabbit Polyclonal Antibody (FITC)
TargetTLR4
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationFITC
ReactivityHuman
Predicted ReactivityBovine, Mouse, Porcine, Rat, Sheep
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human TLR4 (121-200/839aa)
Tested applicationsFC
Dilution rangeFlow-Cyt=1μg/Test
Antibody TypePrimary Antibody
StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Alternative namesTLR-4; TLR 4; ARMD10; CD 284; CD284; CD284 antigen
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NoteFor research use only
TLR4 Rabbit Polyclonal Antibody (FITC)

Blank control: HUVEC. Primary Antibody (green line): Rabbit Anti-TLR4 antibody (orb463501), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

TLR4 Rabbit Polyclonal Antibody (FITC)

Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-TLR4 antibody (orb463501), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

TLR4 Rabbit Polyclonal Antibody (FITC)

Blank control: RAW264.7. Primary Antibody (green line): Rabbit Anti-TLR4 antibody (orb463501), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

TLR4 Rabbit Polyclonal Antibody (FITC)

Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-TLR4 antibody (orb463501), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

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