TLR4 Antibody

• Catalog Number: orb1238646
• Category: Tools
• Description: TLR4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Isotype: IgG
• Immunogen: TLR4 antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of human TLR4.The immunogen is located within amino acids 30 - 80 of TLR4.
• Concentration: 1 mg/mL
• Dilution range: TLR4 antibody can be used for detection of TLR4 by Western blot at 2 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 92 kDa Observed: 94 kDa
• Target: TLR4
• UniProt ID: O00206
• NCBI: NP_612564
• Storage: TLR4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: TLR4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: TLR4 Antibody: TOLL, CD284, TLR-4, ARMD10, Toll-li
• Note: For research use only
• Application notes: TLR4 antibody can be used for detection of TLR4 by Western blot at 2 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TLR4 in human small intestine lysate with TLR4 antibody at (A) 2 and (B) 4 µg/mL.

Immunocytochemistry of TLR4 in K562 cells with TLR4 antibody at 2 µg/mL.

Immunohistochemistry of TLR4 in human spleen tissue with TLR4 antibody at 2.5 µg/ml.

Immunofluorescence of TLR4 in human spleen tissue with TLR4 antibody at 20 µg/ml. Green: TLR4 Antibody (orb1238646) Blue: DAPI staining.