TJP3 Antibody

• Catalog Number: orb1260598
• Category: Antibodies
• Description: TJP3 Antibody
• Target: TJP3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 710-919 of human TJP3 (NP_001254489.1).
• UniProt ID: O95049
• MW: Observed: 150kDa
• Tested applications: IF, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Tight junction protein ZO-3, Tight junction protei
• Note: For research use only

Western blot analysis of extracts of HeLa cells, using TJP3 antibody (orb1260598) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.