Titin Rabbit Polyclonal Antibody

• Catalog Number: orb1205
• Category: Antibodies
• Description: Titin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Titin/CMD1G (2601-2800/34350aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, Flow-Cyt=1ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 3780 kDa
• Target: TTN
• UniProt ID: Q8WZ42
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: TTN; MPRM; Cardiomyopathy dilated 1G(autosomal dom
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Molt-4 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CMD1G Antibody (orb1205) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Paraformaldehyde-fixed, paraffin embedded (Human myocardium), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Titin) Polyclonal Antibody, Unconjugated (orb1205) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.