TIMD4 Antibody

• Catalog Number: orb1240179
• Category: Antibodies
• Description: TIMD4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: TIM-4 antibody was raised against a 16 amino acid synthetic peptide from near the center of human TIM-4.The immunogen is located within amino acids 60 - 110 of TIM-4.
• Concentration: 1 mg/mL
• Dilution range: TIM-4 antibody can be used for the detection of TIM-4 by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 45 kDa
• Target: TIMD4
• UniProt ID: Q96H15
• NCBI: NP_612388
• Storage: TIM-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: TIM-4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: TIM-4 Antibody: TIM4, SMUCKLER, TIM4, T-cell immun
• Note: For research use only
• Application notes: TIM-4 antibody can be used for the detection of TIM-4 by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TIM-4 in RAW264.7 cell lysate with TIM-4 antibody at 2 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry of TIM-4 in Jurkat cells with TIM-4 antibody at 10 µg/mL.

Immunofluorescence of TIM-4 in Jurkat cells with TIM-4 antibody at 20 µg/mL.

Immunohistochemistry of TIM-4 in human spleen tissue with TIM-4 antibody at 2.5 µg/ml.

Immunofluorescence of TIM-4 in human spleen tissue with TIM-4 antibody at 20 µg/ml. Green: TIM-4 Antibody (orb1240179) Blue: DAPI staining.

Immunohistochemistry of TIM-4 in mouse liver tissue with TIM-4 antibody at 2 µg/ml.