Tim-3 antibody

• Catalog Number: orb758816
• Category: Antibodies
• Description: Rat monoclonal antibody to Tim-3
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 2C12
• Tested applications: Blocking, FC, IF, IHC
• Reactivity: Mouse
• Isotype: IgG1
• Immunogen: This antibody was raised by immunising rats with TIM-3:Ig fusion protein emulsified in complete Freund’s adjuvant.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Tim-3
• UniProt ID: Q8VIM0
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD366; HAVcr-2; HAVCR2; Hepatitis A virus cellular
• Note: For research use only
• Application notes: This antibody has been used in multiple FACS analyses for diverse immuno-oncological applications, such as to delineate how TIM-3 signaling on cells of the innate immune system critically influences regulation of the adaptive immune response (Frisancho-Kiss et al, 2006), to demonstrate the reversal of T cell exhaustion and restoration of anti-tumor immunity upon co-targeting Tim-3 and PD-1 pathways (Sakuishi et al, 2010), and to examine the therapeutic potential of dendritic cell based vaccines against malignant glioma (Dey et al, 2015). This antibody has also been used in immunohistochemistry to aid the elucidation of the effects of Th2 immune deviation on corneal allograft survival and possible mechanisms of graft rejection (Beauregard et al, 2005). In addition, employing this anti-TIM-3 antibody as part of the combination immuntherapy (anti-TIM3 + anti-PD-1 +/- anti-CTLA-4) has been shown to be effective against experimental and carcinogen-induced tumors in mice (Sakuishi et al, 2010; Ngiow et al, 2011).
• Expiration Date: 12 months from date of receipt.