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Catalog Number | orb1240153 |
---|---|
Category | Antibodies |
Description | HAVCR2 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 10C10 |
Tested applications | ELISA, FC, ICC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1,k |
Immunogen | TIM-3 antibody was raised against the extracellular domain of human TIM-3 |
Concentration | 1 mg/mL |
Dilution range | TIM-3 antibody can be used for ELISA starting at 0.1 μg/mL. For Western blot start at 1 μg/mL. For Immunocytochemistry start at 1 μg/mL. For Immunofluorescence start at 10 μg/mL. For Immunohistochemistry start at 5 μg/mL. For Flow Cytometry start at 0.1 μg/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 33 kDa Observed: 37 kDa |
Target | HAVCR2 |
UniProt ID | Q8TDQ0 |
NCBI | NP_116171 |
Storage | TIM-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | TIM-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol. |
Alternative names | TIM-3 Antibody: Hepatitis A virus cellular recepto Read more... |
Note | For research use only |
Application notes | TIM-3 antibody can be used for ELISA starting at 0.1 μg/mL. For Western blot start at 1 μg/mL. For Immunocytochemistry start at 1 μg/mL. For Immunofluorescence start at 10 μg/mL. For Immunohistochemistry start at 5 μg/mL. For Flow Cytometry start at 0.1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of TIM-3 in overexpressing HEK293 cells TIM-3 antibody at (A) 0.25, (B) 0.5, and (C) 1 μg/ml
Immunocytochemistry of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
Immunofluorescence of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 10 μg/mL. Green: TIM-3 Antibody [10C10] (orb1240153) Blue: DAPI staining
Immunofluorescence of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 20 μg/mL. Green: TIM-3 Antibody [10C10] (orb1240153) Blue: DAPI staining
Immunohistochemistry of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 5 μg/mL.
Flow cytometry analysis of TIM-3 overexpressing HEK293 cells using TIM-3 antibody and control mouse IgG antibody at 0.1 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIM-3 overexpressing HEK293 cells.
IHC-P, WB | |
Guinea pig, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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