TIAL1 Antibody

• Catalog Number: orb1248920
• Category: Antibodies
• Description: TIAL1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SPEITTEDIKSAFAP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in lysates of cell line K562 (calculated MW of 43.4kDa according to NP_001029097.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Skin shows staining of select keratinocytes. Recommended concentration, 3-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TIAL1
• UniProt ID: Q01085
• NCBI: NP_001029097.1, NP_003243.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TIAL1, TIA1 cytotoxic granule-associated RNA bindi
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in lysates of cell line K562 (calculated MW of 43.4kDa according to NP_001029097.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Skin shows staining of select keratinocytes. Recommended concentration, 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248920 (2 ug/ml) staining of K562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248920 (3.8 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.