TIAL1 Antibody

• Catalog Number: orb1248920
• Category: Antibodies
• Description: TIAL1 Antibody
• Target: TIAL1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-SPEITTEDIKSAFAP
• UniProt ID: Q01085
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in lysates of cell line K562 (calculated MW of 43.4kDa according to NP_001029097.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Skin shows staining of select keratinocytes. Recommended concentration, 3-5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TIAL1, TIA1 cytotoxic granule-associated RNA bindi
• Note: For research use only
• NCBI: NP_001029097.1, NP_003243.1

orb1248920 (2 ug/ml) staining of K562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248920 (3.8 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.