TIA1 Rabbit Polyclonal Antibody

• Catalog Number: orb100996
• Category: Antibodies
• Description: TIA1 Rabbit Polyclonal Antibody
• Target: TIA1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Equine, Mouse, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human TIA1 (101-200/386aa)
• UniProt ID: P31483
• MW: 43 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cytotoxic granule associated RNA binding protein 1
• Note: For research use only

Tissue/cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-TIA1 Polyclonal Antibody, Unconjugated (orb100996) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: rat uterus, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-TIA1 Polyclonal Antibody, Unconjugated (orb100996) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.