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THRB Antibody

SKU: orb345636

Description

Thyroid Hormone Receptor beta 1 antibody

Images & Validation

Tested ApplicationsELISA, IF, IP, WB
Dilution RangeELISA: 1:200,000 – 1:220,000, IF: 1:1000, IP: 1:100, WB: 1:500 - 1:2,000
ReactivityHuman, Mouse
Application Notes
This affinity purified antibody has been tested for use in ELISA, IF, and western blotting.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenThis TR Beta1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human THRB isoform 1 protein.
TargetTHRB
PurityAntibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human THRB protein. A BLAST analysis was used to suggest cross-reactivity with THRB from mouse, human and rat based on a 100% homology with the immunizing sequence. Cross-reactivity with THRB from other sources has not been determined.
ConjugationUnconjugated

Storage & Handling

StorageStore TR B1 antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.66 mg/mL
Expiration Date12 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

Rabbit anti-THRB antibody, rabbit anti-Thyroid hormone receptor beta 1 antibody, Anti-THRB1 antibody, c-erbA-beta, THR1 antibody, c-erbA-2, Nuclear receptor subfamily 1 group A member 2, ERBA2, NR1A2

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeq40806162

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

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25 μl
$ 280.00
Free Secondary Antibody (20 ul)0/0

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