Thyroid Hormone Receptor beta 1 Antibody

SKU: orb345635

Description

Thyroid Hormone Receptor beta 1 antibody

Images & Validation

• Tested Applications: ELISA, IF, IP, WB
• Dilution range: ELISA: 1:20,000 – 1:200,000, IF: 1:1000, IP: 1:100, WB: 1:500 - 1:2,000
• Reactivity: Human, Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, IF, and western blotting.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-TR ß1 antibody is affinity purified from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human THRB isoform 1 protein.
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human THRB protein. A BLAST analysis was used to suggest cross-reactivity with THRB from mouse, human and rat based on a 100% homology with the immunizing sequence. Cross-reactivity with THRB from other sources has not been determined.

Storage & Handling

• Storage: Store Anti-TR beta1 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.15 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Rabbit anti-THRB antibody, rabbit anti-Thyroid hormone receptor beta 1 antibody, Anti-THRB1 antibody, c-erbA-beta, THR1 antibody, c-erbA-2, Nuclear receptor subfamily 1 group A member 2, ERBA2, NR1A2

Immunofluorescence microscopy anti -THRB1 (Thyroid hormone receptor Beta 1) antibody orb345635. Tissue: Mouse Dendritic cells. Primary antibody: Anti THRB1 1:100 1 hr PBS 3% BSA (left) Normal rabbit IgG isotype control (right). Secondary Ab: 488 dye conjugate 1:1000 1 hr. Mounting: Fluoromount-G for examination.

Western blot using Biorbyt's affinity purified anti-THRB1 antibody shows detection of in vitro translated THRB1 (lane 2) and human thyroid stable cell line expressing THTRb1 (lane 3). No staining is evident in non transfected human thyroid cells (lane 1), over expressed THRA (lane 4) or HeLa stable cell line expressing THRA1 (lane 5). The band at ~62 kDa, indicated by the arrowheads, corresponds to THRB.

Quick Database Links

UniProt

P10828

RefSeq:40806162