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Thrombin heavy chain Rabbit Polyclonal Antibody

SKU: orb13715

Description

Thrombin heavy chain Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityMouse
Predicted ReactivityBovine, Equine, Fish, Gallus, Human, Porcine, Rabbit, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Thrombin heavy chain (551-622/622aa)
TargetF2
Molecular Weight28/68 kDa
PurificationAffinity purified by Protein A

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

coagulation factor II; prothrombin; F2; Cf-2; Cf2; FII; F 2; coagulation factor II(thrombin); Coagulation factor II; Coagulation factor II precursor; F2; Factor II; Factor-II; Prothrombin; prothrombin B-chain; PT; serine protease; THRB; THRB_HUMAN; Thrombin; Thrombin heavy chain.

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Thrombin heavy chain Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Thrombin heavy chain) Polyclonal Antibody, Unconjugated (orb13715) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Thrombin heavy chain Rabbit Polyclonal Antibody (orb13715)

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Available Sizes

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50 μl
$ 240.00
100 μl
$ 390.00
200 μl
$ 510.00
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