ThPOK Antibody

• Catalog Number: orb1524346
• Category: Antibodies
• Description: ThPOK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 200 and 250
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ThPOK
• UniProt ID: O15156
• NCBI: NP_056956.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: zinc finger and BTB domain containing 15; ZNF857B;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ThPOK by western blot. Samples: Whole cell lysate (50 µg) from LNCaP, A-549, PC-3, 22Rv1, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ThPOK antibody (orb1524346) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human ThPOK by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from LNCaP cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ThPOK antibody (orb1524346) used for IP at 6 µg per reaction. ThPOK was also immunoprecipitated by a second antibody against a different epitope of ThPOK.