Thioredoxin 2 Rabbit Polyclonal Antibody

SKU: orb4663

Description

Thioredoxin 2 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Hypoxia, Mitochondrial, Mitochondrial Markers

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Thioredoxin 2 (101-166/166aa)
• Target: TXN2
• Molecular Weight: 12 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

mitochondrial thioredoxin; MT-TRX; MTRX; thioredoxin 2 precursor; Thioredoxin mitochondrial; Thioredoxin2; TRX 2; TRX2; TXN 2; TXN2; THIOM_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Thioredoxin 2) Polyclonal Antibody, Unconjugated (orb4663) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Small intestine tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Spleen tissue lysates, Lane 5: Human K562 cell lysates, Lane 6: Human HepG2 cell lysates, Lane 7: Human 293T cell lysates, Lane 8: Human Jurkat cell lysates, Lane 9: Human HeLa cell lysates, Primary: Anti-Thioredoxin 2 (orb4663) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa.

Quick Database Links

Gene Symbol

TXN2

UniProt

Q99757