THBS3 Antibody

• Catalog Number: orb1249545
• Category: Antibodies
• Description: THBS3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: CNDTVPEDFEPFRRQ
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: THBS3
• UniProt ID: P49746
• NCBI: NP_009043.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: THBS3, thrombospondin 3, MGC119564, MGC119565, TSP
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Lung and in lysates of cell line A549 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kDa according to NP_009043.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Colon shows endothelial cell staining of a blood vessel. Recommended concentration, 1-2ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249545 5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.