Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human TGF-beta 3 (321-412/412aa)
Target	TGFB3
Molecular Weight	30/47 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Transforming growth factor beta-3 proprotein; TGFB3; ARVD; FLJ16571; MGC105479; MGC118722; TGF beta 3; TGF beta3; TGF-beta-3; TGF-beta 3; TGFB 3; Transforming growth factor beta-3; TGFB3_HUMAN.

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Blank control (black line): SH-SY5Y. Primary Antibody (green line): Rabbit Anti-TGF-beta 3 antibody (orb158140), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Negative control (white blue line): PBS, Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Ovary (Mouse) Lysate at 40 ug, Primary: Anti-TGF-beta 3 (orb158140) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 30'47kD, Observed band size: 47kD.

Quick Database Links

Gene Symbol

TGFB3

UniProt

P10600

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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