TGF beta 3 propeptide Rabbit Polyclonal Antibody

• Catalog Number: orb11470
• Category: Antibodies
• Description: TGF beta 3 propeptide Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Rabbit
• Reactivity: Human, Mouse, Porcine, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TGF-beta 3 (41-90/412aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 30/47 kDa
• Target: TGFB3
• UniProt ID: P10600
• RRID: AB_10747550
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Transforming growth factor beta-3 proprotein; TGFB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: SHSY5Y. Primary Antibody (green line): Rabbit Anti-TGF beta 3 propeptide antibody (orb11470), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Prostate tissue lysates, Lane 2: Mouse Placenta tissue lysates, Lane 3: Rat Prostate tissue lysates, Lane 4: Rat Placenta tissue lysates, Lane 5: Human HepG2 cell lysates, Lane 6: Human MCF-7 cell lysates, Lane 7: Human SH-SY5Y cell lysates, Primary: Anti-TGF beta 3 propeptide (orb11470) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30/47 kDa, Observed band size: 47 kDa.

Tissue/Cell: pig bile duct, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TGF-beta-3 Polyclonal Antibody, Unconjugated (orb11470) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.