Tfrc Antibody

• Catalog Number: orb1671722
• Category: Antibodies
• Description: Tfrc Antibody
• Clonality: Recombinant
• Clone Number: R17 217.1.3
• Tested applications: Depletion, FC
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: The antibody was obtained from a fusion of the murine myeloma S 194/5.XXO.BU. 1 with spleen cells from a BDIX rat immunized with the Friend erythroleukemia 745.6.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Tfrc
• UniProt ID: Q62351
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD71, Transferrin receptor protein 1, TR, , TfR1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of RAW 264.7 cells using anti-Transferrin receptor antibody (R17 217.1.3). Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric r version of R17 217.1.3 (orb1671722) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671722- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

Flow-cytometry using the Anti-Transferrin receptor antibody R17 217.1.3. RAW 264.7 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r version of R17 217.1.3 (orb1671722 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.