Transferrin Receptor/TFRC Antibody

• Catalog Number: orb215987
• Category: Antibodies
• Description: Transferrin Receptor/TFRC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human TFRC recombinant protein (Position: M1-N198). Human TFRC shares 72% and 70% amino acid (aa) sequences identity with mouse and rat TFRC, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 84871 MW
• UniProt ID: P02786
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Transferrin receptor protein 1;TR;TfR;TfR1;Trfr;T9
• Note: For research use only
• Application notes: WB: The detection limit for TFRC is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-TFRC antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U87 cells using anti-TFRC antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of TFRC using anti-TFRC antibody.Lane 1:human HeLa cell; 2:human K562 cell; 3:human HT1080 cell; 4:rat thymus tissue; 5:rat RAW264.7 cell; 6:mouse SP2/0 cell.

IF analysis of TFRC using anti-TFRC antibody.TFRC was detected in immunocytochemical section of A431 cells.

IF analysis of TFRC using anti-TFRC antibody. TFRC was detected in a paraffin-embedded section of human placenta tissue.

IHC analysis of TFRC using anti-TFRC antibody.TFRC was detected in paraffin-embedded section of human placenta tissue.

IHC analysis of TFRC using anti-TFRC antibody.TFRC was detected in frozen section of human placenta tissues.