TFRC Antibody

• Catalog Number: orb1252162
• Category: Antibodies
• Description: TFRC Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TFRC/1817
• Tested applications: ELISA, FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2b, kappa
• Immunogen: Amino acids 94-212 (extracellular domain) of the human protein were used as the immunogen for the Transferrin Receptor / CD71 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: WB: 0.5-1 ug/mlELISA: 2-4 ug/ml; order the BSA free formulation for coatingFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTOptimal dilution of the CD71 antibody should be determined by the researcher.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TFRC
• UniProt ID: P02786
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Transferrin receptor protein 1, TR, TfR, TfR1, Trf
• Note: For research use only
• Application notes: WB: 0.5-1 ug/mlELISA: 2-4 ug/ml; order the BSA free formulation for coatingFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTOptimal dilution of the CD71 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human placenta tissue with Transferrin Receptor / CD71 antibody (clone TFRC/1817). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human 1) Jurkat, 2) HeLa, 3) MCF7 and K562 cell lysate with CD71 antibody (clone TFRC/1817). Predicted molecular weight: 85-95 kDa.

IHC testing of FFPE human bone marrow with Transferrin Receptor / CD71 antibody (clone TFRC/1817). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free CD71 Antibody (clone TFRC/1817). Confirmation of Integrity and Purity of the Antibody.