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Catalog Number | orb2372527 |
---|---|
Category | Antibodies |
Description | TFCP2C Rabbit Polyclonal Antibody (BF647) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF |
Predicted Reactivity | Bovine, Canine, Equine, Gallus, Human, Mouse, Sheep |
Reactivity | Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human TFCP2C (201-300/502aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | IF=1:50-200 |
Conjugation | BF647 |
Target | TFCP2 |
Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Alpha globin transcription factor CP2; CP2; LBP 1C Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control: DU145. Primary Antibody (green line): Rabbit Anti-TFCP2C antibody (orb2372527), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: DU145. Primary Antibody (green line): Rabbit Anti-TFCP2C antibody (orb2372527), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: DU145. Primary Antibody (green line): Rabbit Anti-TFCP2C antibody (orb2372527), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.