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TFAM Antibody
SKU: orb1260360
Featured
Description
Research Area
Cancer Biology, Cell Biology, Epigenetics & Chromatin, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 10
| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TFAM (NP_003192.1). |
| Target | TFAM |
| Molecular Weight | Observed: 24kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Transcription Factor AntibodiesAM, TCF6, MTTF1, MTTFA, TCF6L1, TCF6L2, TCF6L3
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Western blot analysis of extracts of various cell lines, using TFAM antibody (orb1260360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse spleen using TFAM antibody (orb1260360) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TFAM antibody (orb1260360) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using TFAM antibody (orb1260360) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using TFAM antibody (orb1260360) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TFAM antibody (orb1260360) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using TFAM antibody (orb1260360) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using TFAM antibody (orb1260360) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using TFAM antibody (orb1260360) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SET antibody (orb1260886) at dilution of 1:100 (40x lens).
Quick Database Links
Gene Symbol
TFAM
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
TFAM Antibody (orb1260360)
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