TEV Protease Antibody

SKU: orb344661

Description

TEV Protease antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:1,500-1:27,000, WB: 1:500
• Reactivity: Other
• Application Notes:
  This protein-A purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to TEV by western blotting in the appropriate cell lysate or extract. Anti-TEV protease may be useful to detect residual TEV protease in preparations of recombinant proteins in which that protease may interfere with downstream manipulations.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant MBP-and-poly-His-tagged auto inactivation-resistant mutant TEV Protease.
• Purity: This product was protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. Assay by western blot showed no reactivity to MBP.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.01 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-TEV Protease Antibody, Tobacco Etch Virus, tobacco etch potyvirus, TEV, TEV Protease

Ecoli lysate containing recombinant TEV protease was loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 1% BSA in TBS for 30 min at ambient. The membrane was probed with the primary antibody at a 1:1000 dilution in 1%BSA/TBS overnight at 4°C. For detection HRP conjugated Gt-a-Rabbit IgG (p/n orb347654) was used at a 1:40000 dilution for 30 min at ambient and data generated with FemtoMax™ enhanced chemiluminescent reagent. Expect a band approximately 27 kDa.

Quick Database Links

UniProt

P04517

RefSeq (Protein):NP_062908.1