TET3 Rabbit Polyclonal Antibody

SKU: orb186443

Description

TET3 Rabbit Polyclonal Antibody

Research Area

DNA methylation

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:200-800, Flow-Cyt=2ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TET3 (1561-1660/1660aa)
• Target: TET3
• Molecular Weight: 179 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

hCG_40738; KIAA0401; Methylcytosine dioxygenase TET3; Probable methylcytosine dioxygenase TET3; Tet methylcytosine dioxygenase 3; Tet oncogene family member 3; Tet3; TET3_HUMAN.

Blank control (black line): MCF-7. Primary Antibody (green line): Rabbit Anti-TET3 antibody (orb186443), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

MCF-7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (TET3) polyclonal Antibody, Unconjugated (orb186443) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (TET3) Polyclonal Antibody, Unconjugated (orb186443) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TET3) Polyclonal Antibody, Unconjugated (orb186443) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TET3) Polyclonal Antibody, Unconjugated (orb186443) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) for 90 minutes, and DAPI for nuclei staining.

Quick Database Links

Gene Symbol

TET3

UniProt

O43151